Globally, 1.1 billion people rely on unsafe drinking water sources from lakes, rivers, and open wells. Studies have confirmed that water related diseases not only remain a leading cause of morbidity and mortality worldwide but that the spectrum of disease is expanding and the incidence of many water related microbial diseases are increasing. Thus, this study was conducted to assess contamination level of water and determine the major sources of contaminants at collection points. Three sites of three springs and four sites of river water from Yubdo-Legebatu PA were selected for this study.